Desk studies on feasibility of horizontal standard methods for detection of Clostridium perfringens and Enterococci
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منابع مشابه
Occurrence of Beta2 toxigenic Clostridium perfringens isolates with different toxin types in Iran
Clostridium perfringens is an important cause of enteric diseases in both human and animals. The bacteria produce several toxins which play key roles in the pathogenesis of diseases and are classified into five toxin types, on the basis of the differential production of Alpha, Beta, Epsilon and Iota toxins. In this study a single PCR assay was developed and used for detection of cpb2 gene to id...
متن کاملارزیابی توانایی کلستریدیوم پرفرنژنس در تعیین آلودگی مدفوعی آب در مقایسه با باکتری های شاخص
Introduction & Purpose Fecal coliforms and Escherichia coli have been conventionally used as indicators of microbial pollution of drinking water. The European Directive on drinking water quality has included Clostridium perfringens as one of the microbiological parameters to be determined in control of the water quality for human consumption. This study was carried out to evaluate the efficien...
متن کاملIdentification of enterototxin harboring gene among Clostridium perfringens isolates with different toxin types in Iran
Background: Clostridium perfringens is known as the most widely distributed pathogenic microorganism in nature. It is an extremely important pathogen of human and domestic animals. In a commonly used classification scheme, C. perfringens is divided into five toxinotypes (A to E) based on the production of four major toxins (alpha, beta, epsilon, and iota). Enterotoxin is not usually used for C....
متن کاملDetection and Frequency of Enterotoxin (cpa,cpe) Genes of Clostridium perfringens Isolated from Dehydrated Vegetables by PCR
Background and Objective: Clostridium perfringens is an anaerobic bacterium, commonly present in retail foods. Its enterotoxin-producing ability, short generation time, ability to grow at elevated temperatures, and spore-forming ability, allows it to survive in food-processing temperatures, and cause foodborne illness. The aim of study was to screen dehydrated vegetables contaminated with cpe a...
متن کاملMolecular typing of toxigenic Clostridum perfringens isolated from sheep in Iran
In this research a molecular method based on polymerase chain reaction for typing of Clostridium perfringens was developed and toxin genotypes of 64 isolates from sheep and goats in Iran were determined. The PCR assays were developed for detection of alpha (cpa), beta (cpb) and epsilon (etx) toxin genes, allowing classification of the isolates into genotypes A B, C and D. The field isolates ...
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تاریخ انتشار 2003